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hav quantitative genomic dna  (ATCC)


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    ATCC hav quantitative genomic dna
    Hav Quantitative Genomic Dna, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hav+quantitative+genomic+dna/Quantitative+Synthetic+DNA+from+Hepatitis+A+virus/pm41423617-231-8-13
    Average 94 stars, based on 9 article reviews
    hav quantitative genomic dna - by Bioz Stars, 2026-09
    94/100 stars

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    Reverse Transcription:

    Article Title: Whole genome sequencing of hepatitis A virus: adapting Illumina protocols for foodborne investigation.
    Article Snippet: The tested DNA polymerases included: (1) Thermus aquaticus YT1 Taq polymerase (Bioneer, Daejeon, South Korea), (2) Q5® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA), and (3) Platinum SuperFi II DNA Polymerase (Thermo Fisher Scientific).The tested DNA polymerases included: (1) Thermus aquaticus YT1 Taq polymerase (Bioneer, Daejeon, South Korea), (2) Q5® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA), and (3) Platinum SuperFi II DNA Polymerase (Thermo Fisher Scientific).. To evaluate the detection limits of each polymerase, HAV quantitative genomic DNA (VR3257SD, ATCC) was serially diluted to concentrations ranging from 105 to 107 copies/μL.. PCR reactions were prepared under standardized conditions, with final concentrations, reaction mixtures, and thermal cycling parameters specified in Table 3.PCR reactions were prepared under standardized conditions, with final concentrations, reaction mixtures, and thermal cycling parameters specified in Table 3.

    Article Title: Whole genome sequencing of hepatitis A virus: adapting Illumina protocols for foodborne investigation.
    Article Snippet: The qPCR process was carried out by initially heating to 95 °C for 10 min, followed by 45 cycles of heating at 95 °C for 15 sec, 55 °C for 20 sec, and then 72 °C for 15 sec using a CFX96TM Real-Time PCR system (Bio-Rad, Hercules, CA, USA) and Premix Ex Taq (2X)TM (Takara).The qPCR process was carried out by initially heating to 95 °C for 10 min, followed by 45 cycles of heating at 95 °C for 15 sec, 55 °C for 20 sec, and then 72 °C for 15 sec using a CFX96TM Real-Time PCR system (Bio-Rad, Hercules, CA, USA) and Premix Ex Taq (2X)TM (Takara).. A standard curve for the HAV quantitative genomic DNA (VR-3257SD, ATCC) was used.. The viral copy number was determined by linear regression analysis, which showed a high correlation with an R2 value of 0.99.The viral copy number was determined by linear regression analysis, which showed a high correlation with an R2 value of 0.99.

    Article Title: Whole genome sequencing of hepatitis A virus: adapting Illumina protocols for foodborne investigation
    Article Snippet: The tested DNA polymerases included: (1) Thermus aquaticus YT1 Taq polymerase (Bioneer, Daejeon, South Korea), (2) Q5® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA), and (3) Platinum SuperFi II DNA Polymerase (Thermo Fisher Scientific).The tested DNA polymerases included: (1) Thermus aquaticus YT1 Taq polymerase (Bioneer, Daejeon, South Korea), (2) Q5® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA), and (3) Platinum SuperFi II DNA Polymerase (Thermo Fisher Scientific).. To evaluate the detection limits of each polymerase, HAV quantitative genomic DNA (VR-3257SD, ATCC) was serially diluted to concentrations ranging from 105 to 107 copies/μL.. PCR reactions were prepared under standardized conditions, with final concentrations, reaction mixtures, and thermal cycling parameters specified in Table .PCR reactions were prepared under standardized conditions, with final concentrations, reaction mixtures, and thermal cycling parameters specified in Table .

    Article Title: Whole genome sequencing of hepatitis A virus: adapting Illumina protocols for foodborne investigation
    Article Snippet: The qPCR process was carried out by initially heating to 95 °C for 10 min, followed by 45 cycles of heating at 95 °C for 15 sec, 55 °C for 20 sec, and then 72 °C for 15 sec using a CFX96 TM Real-Time PCR system (Bio-Rad, Hercules, CA, USA) and Premix Ex Taq (2X) TM (Takara Bio Inc., Shiga, Japan).carried out by initially heating to 95 °C for 10 min, followed by 45 cycles of heating at 95 °C for 15 sec, 55 °C for 20 sec, and then 72 °C for 15 sec using a CFX96 TM Real-Time PCR system (Bio-Rad, Hercules, CA, USA) and Premix Ex Taq (2X) TM (Takara Bio Inc., Shiga, Japan). ... A standard curve for the HAV quantitative genomic DNA (VR-3257SD, ATCC) was used.. The viral copy number was determined by linear regression analysis, which showed a high correlation with an R 2 value of 0.99.The viral copy number was determined by linear regression analysis, which showed a high correlation with an R 2 value of 0.99.

    Positive Control:

    Article Title: Whole genome sequencing of hepatitis A virus: adapting Illumina protocols for foodborne investigation.
    Article Snippet: The tested DNA polymerases included: (1) Thermus aquaticus YT1 Taq polymerase (Bioneer, Daejeon, South Korea), (2) Q5® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA), and (3) Platinum SuperFi II DNA Polymerase (Thermo Fisher Scientific).The tested DNA polymerases included: (1) Thermus aquaticus YT1 Taq polymerase (Bioneer, Daejeon, South Korea), (2) Q5® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA), and (3) Platinum SuperFi II DNA Polymerase (Thermo Fisher Scientific).. To evaluate the detection limits of each polymerase, HAV quantitative genomic DNA (VR3257SD, ATCC) was serially diluted to concentrations ranging from 105 to 107 copies/μL.. PCR reactions were prepared under standardized conditions, with final concentrations, reaction mixtures, and thermal cycling parameters specified in Table 3.PCR reactions were prepared under standardized conditions, with final concentrations, reaction mixtures, and thermal cycling parameters specified in Table 3.

    Article Title: Whole genome sequencing of hepatitis A virus: adapting Illumina protocols for foodborne investigation.
    Article Snippet: The qPCR process was carried out by initially heating to 95 °C for 10 min, followed by 45 cycles of heating at 95 °C for 15 sec, 55 °C for 20 sec, and then 72 °C for 15 sec using a CFX96TM Real-Time PCR system (Bio-Rad, Hercules, CA, USA) and Premix Ex Taq (2X)TM (Takara).The qPCR process was carried out by initially heating to 95 °C for 10 min, followed by 45 cycles of heating at 95 °C for 15 sec, 55 °C for 20 sec, and then 72 °C for 15 sec using a CFX96TM Real-Time PCR system (Bio-Rad, Hercules, CA, USA) and Premix Ex Taq (2X)TM (Takara).. A standard curve for the HAV quantitative genomic DNA (VR-3257SD, ATCC) was used.. The viral copy number was determined by linear regression analysis, which showed a high correlation with an R2 value of 0.99.The viral copy number was determined by linear regression analysis, which showed a high correlation with an R2 value of 0.99.

    Article Title: Whole genome sequencing of hepatitis A virus: adapting Illumina protocols for foodborne investigation
    Article Snippet: The tested DNA polymerases included: (1) Thermus aquaticus YT1 Taq polymerase (Bioneer, Daejeon, South Korea), (2) Q5® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA), and (3) Platinum SuperFi II DNA Polymerase (Thermo Fisher Scientific).The tested DNA polymerases included: (1) Thermus aquaticus YT1 Taq polymerase (Bioneer, Daejeon, South Korea), (2) Q5® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA), and (3) Platinum SuperFi II DNA Polymerase (Thermo Fisher Scientific).. To evaluate the detection limits of each polymerase, HAV quantitative genomic DNA (VR-3257SD, ATCC) was serially diluted to concentrations ranging from 105 to 107 copies/μL.. PCR reactions were prepared under standardized conditions, with final concentrations, reaction mixtures, and thermal cycling parameters specified in Table .PCR reactions were prepared under standardized conditions, with final concentrations, reaction mixtures, and thermal cycling parameters specified in Table .

    Article Title: Whole genome sequencing of hepatitis A virus: adapting Illumina protocols for foodborne investigation
    Article Snippet: The qPCR process was carried out by initially heating to 95 °C for 10 min, followed by 45 cycles of heating at 95 °C for 15 sec, 55 °C for 20 sec, and then 72 °C for 15 sec using a CFX96 TM Real-Time PCR system (Bio-Rad, Hercules, CA, USA) and Premix Ex Taq (2X) TM (Takara Bio Inc., Shiga, Japan).carried out by initially heating to 95 °C for 10 min, followed by 45 cycles of heating at 95 °C for 15 sec, 55 °C for 20 sec, and then 72 °C for 15 sec using a CFX96 TM Real-Time PCR system (Bio-Rad, Hercules, CA, USA) and Premix Ex Taq (2X) TM (Takara Bio Inc., Shiga, Japan). ... A standard curve for the HAV quantitative genomic DNA (VR-3257SD, ATCC) was used.. The viral copy number was determined by linear regression analysis, which showed a high correlation with an R 2 value of 0.99.The viral copy number was determined by linear regression analysis, which showed a high correlation with an R 2 value of 0.99.

    Negative Control:

    Article Title: Whole genome sequencing of hepatitis A virus: adapting Illumina protocols for foodborne investigation.
    Article Snippet: The tested DNA polymerases included: (1) Thermus aquaticus YT1 Taq polymerase (Bioneer, Daejeon, South Korea), (2) Q5® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA), and (3) Platinum SuperFi II DNA Polymerase (Thermo Fisher Scientific).The tested DNA polymerases included: (1) Thermus aquaticus YT1 Taq polymerase (Bioneer, Daejeon, South Korea), (2) Q5® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA), and (3) Platinum SuperFi II DNA Polymerase (Thermo Fisher Scientific).. To evaluate the detection limits of each polymerase, HAV quantitative genomic DNA (VR3257SD, ATCC) was serially diluted to concentrations ranging from 105 to 107 copies/μL.. PCR reactions were prepared under standardized conditions, with final concentrations, reaction mixtures, and thermal cycling parameters specified in Table 3.PCR reactions were prepared under standardized conditions, with final concentrations, reaction mixtures, and thermal cycling parameters specified in Table 3.

    Article Title: Whole genome sequencing of hepatitis A virus: adapting Illumina protocols for foodborne investigation.
    Article Snippet: The qPCR process was carried out by initially heating to 95 °C for 10 min, followed by 45 cycles of heating at 95 °C for 15 sec, 55 °C for 20 sec, and then 72 °C for 15 sec using a CFX96TM Real-Time PCR system (Bio-Rad, Hercules, CA, USA) and Premix Ex Taq (2X)TM (Takara).The qPCR process was carried out by initially heating to 95 °C for 10 min, followed by 45 cycles of heating at 95 °C for 15 sec, 55 °C for 20 sec, and then 72 °C for 15 sec using a CFX96TM Real-Time PCR system (Bio-Rad, Hercules, CA, USA) and Premix Ex Taq (2X)TM (Takara).. A standard curve for the HAV quantitative genomic DNA (VR-3257SD, ATCC) was used.. The viral copy number was determined by linear regression analysis, which showed a high correlation with an R2 value of 0.99.The viral copy number was determined by linear regression analysis, which showed a high correlation with an R2 value of 0.99.

    Article Title: Whole genome sequencing of hepatitis A virus: adapting Illumina protocols for foodborne investigation
    Article Snippet: The tested DNA polymerases included: (1) Thermus aquaticus YT1 Taq polymerase (Bioneer, Daejeon, South Korea), (2) Q5® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA), and (3) Platinum SuperFi II DNA Polymerase (Thermo Fisher Scientific).The tested DNA polymerases included: (1) Thermus aquaticus YT1 Taq polymerase (Bioneer, Daejeon, South Korea), (2) Q5® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA), and (3) Platinum SuperFi II DNA Polymerase (Thermo Fisher Scientific).. To evaluate the detection limits of each polymerase, HAV quantitative genomic DNA (VR-3257SD, ATCC) was serially diluted to concentrations ranging from 105 to 107 copies/μL.. PCR reactions were prepared under standardized conditions, with final concentrations, reaction mixtures, and thermal cycling parameters specified in Table .PCR reactions were prepared under standardized conditions, with final concentrations, reaction mixtures, and thermal cycling parameters specified in Table .

    Article Title: Whole genome sequencing of hepatitis A virus: adapting Illumina protocols for foodborne investigation
    Article Snippet: The qPCR process was carried out by initially heating to 95 °C for 10 min, followed by 45 cycles of heating at 95 °C for 15 sec, 55 °C for 20 sec, and then 72 °C for 15 sec using a CFX96 TM Real-Time PCR system (Bio-Rad, Hercules, CA, USA) and Premix Ex Taq (2X) TM (Takara Bio Inc., Shiga, Japan).carried out by initially heating to 95 °C for 10 min, followed by 45 cycles of heating at 95 °C for 15 sec, 55 °C for 20 sec, and then 72 °C for 15 sec using a CFX96 TM Real-Time PCR system (Bio-Rad, Hercules, CA, USA) and Premix Ex Taq (2X) TM (Takara Bio Inc., Shiga, Japan). ... A standard curve for the HAV quantitative genomic DNA (VR-3257SD, ATCC) was used.. The viral copy number was determined by linear regression analysis, which showed a high correlation with an R 2 value of 0.99.The viral copy number was determined by linear regression analysis, which showed a high correlation with an R 2 value of 0.99.

    Virus:

    Article Title: Whole genome sequencing of hepatitis A virus: adapting Illumina protocols for foodborne investigation.
    Article Snippet: The tested DNA polymerases included: (1) Thermus aquaticus YT1 Taq polymerase (Bioneer, Daejeon, South Korea), (2) Q5® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA), and (3) Platinum SuperFi II DNA Polymerase (Thermo Fisher Scientific).The tested DNA polymerases included: (1) Thermus aquaticus YT1 Taq polymerase (Bioneer, Daejeon, South Korea), (2) Q5® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA), and (3) Platinum SuperFi II DNA Polymerase (Thermo Fisher Scientific).. To evaluate the detection limits of each polymerase, HAV quantitative genomic DNA (VR3257SD, ATCC) was serially diluted to concentrations ranging from 105 to 107 copies/μL.. PCR reactions were prepared under standardized conditions, with final concentrations, reaction mixtures, and thermal cycling parameters specified in Table 3.PCR reactions were prepared under standardized conditions, with final concentrations, reaction mixtures, and thermal cycling parameters specified in Table 3.

    Article Title: Whole genome sequencing of hepatitis A virus: adapting Illumina protocols for foodborne investigation.
    Article Snippet: The qPCR process was carried out by initially heating to 95 °C for 10 min, followed by 45 cycles of heating at 95 °C for 15 sec, 55 °C for 20 sec, and then 72 °C for 15 sec using a CFX96TM Real-Time PCR system (Bio-Rad, Hercules, CA, USA) and Premix Ex Taq (2X)TM (Takara).The qPCR process was carried out by initially heating to 95 °C for 10 min, followed by 45 cycles of heating at 95 °C for 15 sec, 55 °C for 20 sec, and then 72 °C for 15 sec using a CFX96TM Real-Time PCR system (Bio-Rad, Hercules, CA, USA) and Premix Ex Taq (2X)TM (Takara).. A standard curve for the HAV quantitative genomic DNA (VR-3257SD, ATCC) was used.. The viral copy number was determined by linear regression analysis, which showed a high correlation with an R2 value of 0.99.The viral copy number was determined by linear regression analysis, which showed a high correlation with an R2 value of 0.99.

    Article Title: Whole genome sequencing of hepatitis A virus: adapting Illumina protocols for foodborne investigation
    Article Snippet: The tested DNA polymerases included: (1) Thermus aquaticus YT1 Taq polymerase (Bioneer, Daejeon, South Korea), (2) Q5® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA), and (3) Platinum SuperFi II DNA Polymerase (Thermo Fisher Scientific).The tested DNA polymerases included: (1) Thermus aquaticus YT1 Taq polymerase (Bioneer, Daejeon, South Korea), (2) Q5® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA), and (3) Platinum SuperFi II DNA Polymerase (Thermo Fisher Scientific).. To evaluate the detection limits of each polymerase, HAV quantitative genomic DNA (VR-3257SD, ATCC) was serially diluted to concentrations ranging from 105 to 107 copies/μL.. PCR reactions were prepared under standardized conditions, with final concentrations, reaction mixtures, and thermal cycling parameters specified in Table .PCR reactions were prepared under standardized conditions, with final concentrations, reaction mixtures, and thermal cycling parameters specified in Table .

    Article Title: Whole genome sequencing of hepatitis A virus: adapting Illumina protocols for foodborne investigation
    Article Snippet: The qPCR process was carried out by initially heating to 95 °C for 10 min, followed by 45 cycles of heating at 95 °C for 15 sec, 55 °C for 20 sec, and then 72 °C for 15 sec using a CFX96 TM Real-Time PCR system (Bio-Rad, Hercules, CA, USA) and Premix Ex Taq (2X) TM (Takara Bio Inc., Shiga, Japan).carried out by initially heating to 95 °C for 10 min, followed by 45 cycles of heating at 95 °C for 15 sec, 55 °C for 20 sec, and then 72 °C for 15 sec using a CFX96 TM Real-Time PCR system (Bio-Rad, Hercules, CA, USA) and Premix Ex Taq (2X) TM (Takara Bio Inc., Shiga, Japan). ... A standard curve for the HAV quantitative genomic DNA (VR-3257SD, ATCC) was used.. The viral copy number was determined by linear regression analysis, which showed a high correlation with an R 2 value of 0.99.The viral copy number was determined by linear regression analysis, which showed a high correlation with an R 2 value of 0.99.

    Activity Assay:

    Article Title: Whole genome sequencing of hepatitis A virus: adapting Illumina protocols for foodborne investigation.
    Article Snippet: The tested DNA polymerases included: (1) Thermus aquaticus YT1 Taq polymerase (Bioneer, Daejeon, South Korea), (2) Q5® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA), and (3) Platinum SuperFi II DNA Polymerase (Thermo Fisher Scientific).The tested DNA polymerases included: (1) Thermus aquaticus YT1 Taq polymerase (Bioneer, Daejeon, South Korea), (2) Q5® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA), and (3) Platinum SuperFi II DNA Polymerase (Thermo Fisher Scientific).. To evaluate the detection limits of each polymerase, HAV quantitative genomic DNA (VR3257SD, ATCC) was serially diluted to concentrations ranging from 105 to 107 copies/μL.. PCR reactions were prepared under standardized conditions, with final concentrations, reaction mixtures, and thermal cycling parameters specified in Table 3.PCR reactions were prepared under standardized conditions, with final concentrations, reaction mixtures, and thermal cycling parameters specified in Table 3.

    Article Title: Whole genome sequencing of hepatitis A virus: adapting Illumina protocols for foodborne investigation.
    Article Snippet: The qPCR process was carried out by initially heating to 95 °C for 10 min, followed by 45 cycles of heating at 95 °C for 15 sec, 55 °C for 20 sec, and then 72 °C for 15 sec using a CFX96TM Real-Time PCR system (Bio-Rad, Hercules, CA, USA) and Premix Ex Taq (2X)TM (Takara).The qPCR process was carried out by initially heating to 95 °C for 10 min, followed by 45 cycles of heating at 95 °C for 15 sec, 55 °C for 20 sec, and then 72 °C for 15 sec using a CFX96TM Real-Time PCR system (Bio-Rad, Hercules, CA, USA) and Premix Ex Taq (2X)TM (Takara).. A standard curve for the HAV quantitative genomic DNA (VR-3257SD, ATCC) was used.. The viral copy number was determined by linear regression analysis, which showed a high correlation with an R2 value of 0.99.The viral copy number was determined by linear regression analysis, which showed a high correlation with an R2 value of 0.99.

    Article Title: Whole genome sequencing of hepatitis A virus: adapting Illumina protocols for foodborne investigation
    Article Snippet: The tested DNA polymerases included: (1) Thermus aquaticus YT1 Taq polymerase (Bioneer, Daejeon, South Korea), (2) Q5® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA), and (3) Platinum SuperFi II DNA Polymerase (Thermo Fisher Scientific).The tested DNA polymerases included: (1) Thermus aquaticus YT1 Taq polymerase (Bioneer, Daejeon, South Korea), (2) Q5® High-Fidelity DNA Polymerase (New England Biolabs, Ipswich, MA, USA), and (3) Platinum SuperFi II DNA Polymerase (Thermo Fisher Scientific).. To evaluate the detection limits of each polymerase, HAV quantitative genomic DNA (VR-3257SD, ATCC) was serially diluted to concentrations ranging from 105 to 107 copies/μL.. PCR reactions were prepared under standardized conditions, with final concentrations, reaction mixtures, and thermal cycling parameters specified in Table .PCR reactions were prepared under standardized conditions, with final concentrations, reaction mixtures, and thermal cycling parameters specified in Table .

    Article Title: Whole genome sequencing of hepatitis A virus: adapting Illumina protocols for foodborne investigation
    Article Snippet: The qPCR process was carried out by initially heating to 95 °C for 10 min, followed by 45 cycles of heating at 95 °C for 15 sec, 55 °C for 20 sec, and then 72 °C for 15 sec using a CFX96 TM Real-Time PCR system (Bio-Rad, Hercules, CA, USA) and Premix Ex Taq (2X) TM (Takara Bio Inc., Shiga, Japan).carried out by initially heating to 95 °C for 10 min, followed by 45 cycles of heating at 95 °C for 15 sec, 55 °C for 20 sec, and then 72 °C for 15 sec using a CFX96 TM Real-Time PCR system (Bio-Rad, Hercules, CA, USA) and Premix Ex Taq (2X) TM (Takara Bio Inc., Shiga, Japan). ... A standard curve for the HAV quantitative genomic DNA (VR-3257SD, ATCC) was used.. The viral copy number was determined by linear regression analysis, which showed a high correlation with an R 2 value of 0.99.The viral copy number was determined by linear regression analysis, which showed a high correlation with an R 2 value of 0.99.



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    hav quantitative genomic dna  (ATCC)
    94
    ATCC hav quantitative genomic dna
    Hav Quantitative Genomic Dna, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hav+quantitative+genomic+dna/Quantitative+Synthetic+DNA+from+Hepatitis+A+virus/pm41423617-231-8-13
    Average 94 stars, based on 1 article reviews
    hav quantitative genomic dna - by Bioz Stars, 2026-09
    94/100 stars
    		  Buy from Supplier

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